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KMID : 0613820120220020200
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Journal of Life Science
2012 Volume.22 No. 2 p.200 ~ p.208
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Optimization of Medium Composition for the Mycelial Growth of Sparassis crispa
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Kim Jin-Woo
Cheon Woo-Jae
Chai Kyung-Hee
Kim Dong-Gwan
Son Sung-Ho
Kim Jong-Guk
Lim Hee-Jae
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Abstract
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The characteristics of mycelium growth of Sparassis crispa KGFS08 and KFRI746 in liquid culture were investigated. The optimum growth of the mycelium of S. crispa was observed in the KTM medium. The best carbon source was starch. In terms of nitrogen sources, tryptone affected mycelial growth in the liquid culture. The optimal culture conditions were pH 4.0-5.0 in STK medium [3% (w/v) starch, 0.3% (w/v) tryptone, 0.1% (w/v) KH₂PO₂, and 0.1% (w/v) folic acid].
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KEYWORD
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Sparassis crispa, mycelial growth, liquid culture, carbon source, nitrogen source
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